John Woodruff (February 12, 1826 – May 20, 1868) was a U.S. Representative from Connecticut.
Born in West Hartford, Connecticut, Woodruff received a limited schooling. He moved to Catskill, New York, in 1835. He returned to Connecticut in 1841 and settled in Bristol, where he worked in a clock factory until 1845. He moved to New Haven in 1845.
Woodruff was elected a member of the common council in 1848 and served several terms. He served as member of the general assembly in 1852.
Woodruff was elected as a candidate of the American Party to the Thirty-fourth Congress (March 4, 1855 – March 3, 1857). He was an unsuccessful candidate for reelection in 1856 to the Thirty-fifth Congress.
Woodruff was elected as a Republican to the Thirty-sixth Congress (March 4, 1859 – March 3, 1861). Upon establishment of the office in 1862 was appointed collector of internal revenue for the second district of Connecticut and served until his death in New Haven, Connecticut, May 20, 1868. He was interred in Evergreen Cemetery.