EC number 6.1.1.5 IntEnz IntEnz view ExPASy NiceZyme view | CAS number 9030-96-0 BRENDA BRENDA entry KEGG KEGG entry | |
In enzymology, an isoleucine-tRNA ligase (EC 6.1.1.5) is an enzyme that catalyzes the chemical reaction
ATP + L-isoleucine + tRNAIleThe 3 substrates of this enzyme are ATP, L-isoleucine, and tRNA(Ile), whereas its 3 products are AMP, diphosphate, and L-isoleucyl-tRNA(Ile).
This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-isoleucine:tRNAIle ligase (AMP-forming). Other names in common use include isoleucyl-tRNA synthetase, isoleucyl-transfer ribonucleate synthetase, isoleucyl-transfer RNA synthetase, isoleucine-transfer RNA ligase, isoleucine-tRNA synthetase, and isoleucine translase. This enzyme participates in valine, leucine and isoleucine biosynthesis and aminoacyl-trna biosynthesis.
Structural studies
As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1FFY, 1JZQ, 1JZS, 1QU2, 1QU3, 1UDZ, 1UE0, 1WK8, 1WNY, and 1WNZ.