Inorganic diphosphatase

In enzymology, an inorganic diphosphatase (EC 3.6.1.1) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are diphosphate and H₂O, whereas its product is phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is diphosphate phosphohydrolase. This enzyme participates in oxidative phosphorylation. It employs one cofactor, metal.