Hydrogenobyrinic acid a,c diamide synthase (glutamine hydrolysing)

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction

2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H₂O ⇌ 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

The 4 substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H₂O, whereas its 4 products are ADP, phosphate, hydrogenobyrinic acid a,c-diamide, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming). This enzyme is also called CobB. This enzyme participates in porphyrin and chlorophyll metabolism.