Hope-Johnstone is a surname. Notable people with the surname include:
John Hope-Johnstone (1796–1876) of Annandale, Scottish Tory politician
John Hope-Johnstone (1842–1912), Scottish Conservative Party politician
Percy Wentworth Hope-Johnstone (1909–1983), de jure British peer and soldier in the British Army
William James Hope-Johnstone KCB (1798–1878), Royal Navy officer, became Commander-in-Chief, The Nore
James Hope-Johnstone, 3rd Earl of Hopetoun (1741–1816), Scottish Representative Peer
