Holford is a surname. Notable people with the surname include:
Castello Holford, American author of Aristopia
David Holford (born 1940), West Indian cricketer
Karen Holford, Welsh engineer and academic
John Holford (1909-1997), Royal Navy medical officer
Michael Holford (born 1983), English rugby player
Patrick Holford, controversial British nutritionist
Robert Stayner Holford (1808-1892), British politician
Thomas Holford (1541-1588), Catholic priest and martyr
Tom Holford (1878-1964), English footballer
William Holford, Baron Holford (1907-1975), British town planner
