Gyllenstierna is a Swedish surname that may refer to
Ebbe Gyllenstierna (1911–2003), Swedish modern pentathlete
Christina Gyllenstierna (1494/5–1559), wife of Sten Sture the Younger and Swedish resistance leader
Görwel Gyllenstierna (1646–1708), Swedish noblewoman
Johan Göransson Gyllenstierna (1635–1680), Swedish statesman
Karin Gyllenstierna, 17th century Swedish courtier
Maria Gustava Gyllenstierna (1672–1737), Swedish countess, writer and translator
Margareta Gyllenstierna (1689–1740), Swedish countess
Eric Gyllenstierna (1882–1940), Swedish diplomat
