Guillén or Guillen is a Spanish or French surname. Notable people with the surname include:
Ambrosio Guillen (1929–1953), United States Marine who was posthumously awarded the Medal of Honor
Carlos Guillén, retired Venezuelan baseball player
Fernando Guillén (disambiguation), multiple people
Jorge Guillén, Spanish poet
José Guillén (born 1976), Dominican baseball player
Laurice Guillén (born 1947), Filipina actress
Rafael Guillen ("Subcomandante Marcos"), spokesman for the Zapatista Army of National Liberation
Nancy Guillén (born 1976), retired Salvadoran hammer thrower
Néstor Guillén Olmos (1890–1966), President of Bolivia for 27 days
Nicolás Guillén, Afro-Cuban poet
Ozzie Guillén (born 1964), Venezuelan baseball coach and former player
Ricardo Guillén (born 1967), Spanish basketball player
Sammy Guillen (1924–2013), West Indian and New Zealand cricket player
Sara Sotillo (maternal name Guillén, 1900–1961), Panamanian academic and activist