Gellan lyase

Gellan lyase (EC 4.2.2.25) is an enzyme with systematic name gellan beta-D-glucopyranosyl-(1->4)-D-glucopyranosyluronate lyase. This enzyme catalyses the following chemical reaction

Eliminative cleavage of beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4,5-unsaturated D-glucopyranosyluronic acid at the non-reducing end.

The tetrasaccharide produced from deacetylated gellan is beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp.