Florio is a surname. Notable people with the surname include:
James Florio (born 1937), 49th Governor of New JerseyJohn Florio (1553–1625), linguist and lexicographerMassimo Florio, Professor of Public Economics and Chair of Economics of European Integration at the University of MilanMichelangelo Florio (1515–1572) Italian Franciscan friar convert to Protestantism, pastor in England and Switzerland, and father of John FlorioRudy Florio (born 1950), Canadian football playerVincenzo Florio (1883–1959), Italian industrialist and founder of two automobile races