Florio is a surname. Notable people with the surname include:
James Florio (born 1937), 49th Governor of New Jersey
John Florio (1553–1625), linguist and lexicographer
Massimo Florio, Professor of Public Economics and Chair of Economics of European Integration at the University of Milan
Michelangelo Florio (1515–1572) Italian Franciscan friar convert to Protestantism, pastor in England and Switzerland, and father of John Florio
Rudy Florio (born 1950), Canadian football player
Vincenzo Florio (1883–1959), Italian industrialist and founder of two automobile races
