Flavonoid 3',5' hydroxylase

Flavanoid 3',5'-hydroxylase (EC 1.14.13.88) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction

flavanone + 2 NADPH + 2 H⁺ + 2 O₂ ⇌ 3',5'-dihydroxyflavanone + 2 NADP⁺ + 2 H₂O (overall reaction) (1a) flavanone + NADPH + H⁺ + O₂ ⇌ 3'-hydroxyflavanone + NADP⁺ + H₂O (1b) 3'-hydroxyflavanone + NADPH + H⁺ + O₂ ⇌ 3',5'-dihydroxyflavanone + NADP⁺ + H₂O

Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).