Epi isozizaene 5 monooxygenase

Epi-isozizaene 5-monooxygenase (EC 1.14.13.106, CYP170A1) is an enzyme with systematic name (+)-epi-isozizaene,NADPH:oxygen oxidoreductase (5-hydroxylating). This enzyme catalyses the following chemical reaction

(+)-epi-isozizaene + 2 NADPH + 2 H⁺ + 2 O₂ ⇌ albaflavenone + 2 NADP⁺ + 3 H₂O (overall reaction)(1a) (+)-epi-isozizaene + NADPH + H⁺ + O₂ ⇌ (5S)-albaflavenol + NADP⁺ + H₂O(1b) (5S)-albaflavenol + NADPH + H⁺ + O₂ ⇌ albaflavenone + NADP⁺ + 2 H₂O(2a) (+)-epi-isozizaene + NADPH + H⁺ + O₂ ⇌ (5R)-albaflavenol + NADP⁺ + H₂O(2b) (5R)-albaflavenol + NADPH + H⁺ + O₂ ⇌ albaflavenone + NADP⁺ + 2 H₂O

This cytochrome P450 enzyme is purified from bacterium Streptomyces coelicolor.