Doolittle is a surname. Notable people with the surname include:
Amos Doolittle (1754–1832), American engraver and silversmith
Benjamin Doolittle (1825–1895), New York politician
Bev Doolittle (born 1947), American painter
Bill Doolittle (born c. 1921), American former football player and coach
Charles Doolittle (1832–1903), American Civil War general
Dudley Doolittle (1881–1957), American congressman from Kansas
Eliza Doolittle (singer) (born 1988), British singer
Ford Doolittle (born 1942), American biochemist
Hilda Doolittle (1886–1961), American poet
Jimmy Doolittle, American aviation pioneer, general and recipient of the Medal of Honor
James Rood Doolittle (1815–1897), American politician
John Doolittle (born 1950), American congressman from California
Justus Doolittle (1824–1880), American Board of Commissioners for Foreign Missions missionary to Fuhchau, China
Melinda Doolittle (born 1977), American singer
Russell Doolittle (born 1931), American biochemist
Sean Doolittle, American baseball player
Sean Doolittle (author), American novelist
Thomas Doolittle (c. 1632 – 1707), English nonconformist minister, tutor and author
William H. Doolittle (1848–1914), American congressman from Washington State
Fictional characters:
Doctor Dolittle
Eliza Doolittle, main character of the play Pygmalion and its musical adaptation, My Fair Lady
