The Denison, Bonham and New Orleans Railroad was a railroad company based in Denison, Texas, U.S.A. which was chartered in 1887. It was nicknamed "Nellie". The DB&NO operated on track between Bonham Junction on the Missouri-Kansas-Texas Railroad east of Denison and its southeastern terminus with the Texas and Pacific Railway in Bonham, Texas. It made stops in the communities of Ambrose and Ravenna. The railroad ceased operations in 1928.
The track through Bonham was completed in 1891. The railroad ended up totaling 26.7 miles (43.0 km) of track. The Missouri-Kansas-Texas Railroad operated the railroad and later leased it. In 1923 the M-K-T gave up its interest in the line and for two years was operated under receivership. In 1925, several Bonham citizens purchased the line but in 1928 the line was shut down and abandoned.