D'Amboise is a French surname meaning "of Amboise". Notable people with the surname include the following:
Charlotte d'Amboise, Broadway dancer and daughter of Jacques d'Amboise.
Christopher d'Amboise, former New York City Ballet principal dancer and son of Jacques d'Amboise.
Jacques d'Amboise (doctor) (1559–1606), French doctor and surgeon
Jacques d'Amboise (bishop) (ca. 1445–1516), French religious dignitary and patron of medieval France
Jacques d'Amboise (dancer) (born 1934), American ballet dancer and choreographer