Cyclohexyl isocyanide hydratase

In enzymology, a cyclohexyl-isocyanide hydratase (EC 4.2.1.103) is an enzyme that catalyzes the chemical reaction

N-cyclohexylformamide ⇌ cyclohexyl isocyanide + H₂O

Hence, this enzyme has one substrate, N-cyclohexylformamide, and two products, cyclohexyl isocyanide and H₂O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming). Other names in common use include isonitrile hydratase, and N-cyclohexylformamide hydro-lyase. This enzyme participates in caprolactam degradation.