Cucurbitacin Delta23 reductase

In enzymology, a cucurbitacin Delta23-reductase (EC 1.3.1.5) is an enzyme that catalyzes the chemical reaction

23,24-dihydrocucurbitacin + NAD(P)+ ⇌ cucurbitacin + NAD(P)H + H⁺

The 3 substrates of this enzyme are 23,24-dihydrocucurbitacin, NAD⁺, and NADP⁺, whereas its 4 products are cucurbitacin, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 23,24-dihydrocucurbitacin:NAD(P)+ Delta23-oxidoreductase. This enzyme is also called NAD(P)H: cucurbitacin B Delta23-oxidoreductase. It employs one cofactor, manganese.