Samiksha Jaiswal (Editor)

Cryo scanning electron microscopy

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Cryo-scanning electron microscopy (CSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen. Cryo-SEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.

References

Cryo-scanning electron microscopy Wikipedia


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