Crossover junction endodeoxyribonuclease

Crossover junction endodeoxyribonuclease (EC 3.1.22.4, Hje endonuclease, Holliday junction endonuclease CCE1, Holliday junction resolvase, Holliday junction-cleaving endonuclease, Holliday junction-resolving endoribonuclease, RusA Holliday junction resolvase, RusA endonuclease, RuvC endonuclease, SpCCe1 Holliday junction resolvase, crossover junction endoribonuclease, cruciform-cutting endonuclease, endo X3, endonuclease RuvC, endonuclease VII, endonuclease X3, resolving enzyme CCE1) is an enzyme. This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)

The enzyme from Saccharomyces cerevisiae is not active on DNA molecules that do not contain Holliday junctions.