Cholest 4 en 3 one 26 monooxygenase

Cholest-4-en-3-one 26-monooxygenase (EC 1.14.13.141, CYP125, CYP125A1, cholest-4-en-3-one 27-monooxygenase) is an enzyme with systematic name cholest-4-en-3-one,NADH:oxygen oxidoreductase (26-hydroxylating). This enzyme catalyses the following chemical reaction

cholest-4-en-3-one + NADH + H⁺ + O₂ ⇌ 26-hydroxycholest-4-en-3-one + NAD⁺ + H₂O

Cholest-4-en-3-one 26-monooxygenase is a heme thiolate (P450) enzyme.