Bos is a Dutch surname meaning "woods" or "forest". Notable people with the surname include:
Bert Bos, Dutch computer scientist
Hans Bos (born 1950), Dutch biochemist
Henk Bos (disambiguation)
Jan Bos (born 1975), Dutch skater & cyclist
Jan Just Bos (1939–2003), Dutch botanist, television presenter and rower
Jan Ritzema Bos (1850–1928), Dutch phytopathologist
Johnny Bos (1952–2013), American boxer and writer
Lambert Bos (1670–1717), Dutch scholar and critic
Lex Bos (born 1957), Dutch field hockey player
Mark Bos (born 1960), Australian-rules footballer
Nel Bos (born 1947), Dutch swimmer
Ricardo van den Bos (born 1984), Dutch Muay Thai fighter
Stef Bos (born 1961), Dutch singer
Theo Bos (born 1983), Dutch cyclist
Theo Bos (footballer) (1965–2013), Dutch footballer and manager
Wouter Bos (born 1963), Dutch politician, leader of the Labour Party, PvdA