Biotin synthase

In enzymology, a biotin synthase (EC 2.8.1.6) is an enzyme that catalyzes the chemical reaction

dethiobiotin + sulfur + 2 S-adenosyl-L-methionine ⇌ biotin + 2 L-methionine + 2 5'-deoxyadenosine

The 3 substrates of this enzyme are dethiobiotin, sulfur, and S-adenosyl-L-methionine, whereas its 3 products are biotin, L-methionine, and 5'-deoxyadenosine.

This radical SAM enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is dethiobiotin:sulfur sulfurtransferase. This enzyme participates in biotin metabolism. It employs one cofactor, iron-sulfur.