Bertolotti is an Italian surname. Notable people with the surname include:
Alessandro Bertolotti (born 1960), Italian writer and photographer
Andrés Bertolotti (born 1943), Argentine footballer
Bernardino Bertolotti, 16th-century Italian composer and musician
Cesare Bertolotti (1854–1932), Italian painter
Gianni Bertolotti (born 1950), Italian basketball player
Giovanni Lorenzo Bertolotti (1640–1721), Italian Baroque painter
Mariano Bertolotti (born 1982), Argentine judoka