Bertolini is an Italian surname. Notable people with the surname include:
Adrián Bertolini (born 1978), Uruguayan basketball playerAlessandro Bertolini, cyclistAndrea Bertolini, race car driverChristopher Bertolini, American film writer and producerGioele Bertolini (born 1995), Italian cyclistGiuseppe Bertolini, botanistJack Bertolini (born 1934), Scottish former professional footballerJosé Elguero Bertolini (born 1934), Spanish chemistLuigi Bertolini, football playerMark Bertolini (born 1956), CEO of AetnaMassimo Bertolini, tennis playerMichelle Bertolini (born 1994), Venezuelan tennis playerPietro Bertolini, Italian statesmanRoberto Bertolini (born 1985), Italian javelin throwerVeronica Bertolini, Italian gymnast