Bertolini is an Italian surname. Notable people with the surname include:
Adrián Bertolini (born 1978), Uruguayan basketball player
Alessandro Bertolini, cyclist
Andrea Bertolini, race car driver
Christopher Bertolini, American film writer and producer
Gioele Bertolini (born 1995), Italian cyclist
Giuseppe Bertolini, botanist
Jack Bertolini (born 1934), Scottish former professional footballer
José Elguero Bertolini (born 1934), Spanish chemist
Luigi Bertolini, football player
Mark Bertolini (born 1956), CEO of Aetna
Massimo Bertolini, tennis player
Michelle Bertolini (born 1994), Venezuelan tennis player
Pietro Bertolini, Italian statesman
Roberto Bertolini (born 1985), Italian javelin thrower
Veronica Bertolini, Italian gymnast
