The Atlantic, Suwannee River and Gulf Railroad Company was incorporated under the general incorporation laws of Florida in 1893.
Florida state law chapter 4267, approved May 24, 1893, incorporated the Atlantic, Suwannee River and Gulf Railway Company. It was owned by Comer L. Peek, F. L. Babbitt, Thomas P. Denham, George Tompsett and J. G. Alvarez.
It was established to build, construct, maintain, manage and operate one of more lines of railway, as follows: Beginning at or near the town of Starke, in the county of Bradford, Florida, and extending from thence through the counties of Bradford and Alachua to a point on or near the Suwannee River between Clay Landing and Fort White, and a branch or branches through the counties of Levy, Columbia, Lafayette and Taylor, and with a branch or branches through the counties of Bradford, Clay and Duval, to a point on the St. Johns river.
The company had the right to build canals as feeders for the railroad, which was to be standard gauge. The railroad was promised land grants along its line.
The company merged with the already-incorporated Atlantic, Suwannee River and Gulf Railroad Company, which had begun to build the line.