Antonina Prusinowska, or Pruszanowska, or Prosinowska (fl. 1767), was a Polish stage actress. She was the first native actress in Poland.
Antonina Prusinowska was employed as a member of the pioneer group of actors engaged upon the foundation of the National Theatre, Warsaw. Prior to this, only foreign theater companies, normally French and Italian, had been active in Poland, and the foundation of the national theater also signified the start point of the first professional Polish theater, and the actors employed at the newly founded theater was thus the first native professional actors in Poland. With no training, they were tutored by the theater direction, the playwrights and enthusiastic noble amateurs.
Antonina Prusinowska participated in the inauguration performance of the National Theatre, Natrętów, on 19 November 1765, likely in the role of Lucyndę. Thus, she and her female colleague, Wiktoria Leszczyńska, became historic as the first native actresses in Poland. A critic mentioned, that the two actresses were both noblewomen who had abandoned their husbands, and that they both acted well, if somewhat hesitant. They were both active at the theater for two years. Prusinowska were given the highest salary as the theater's leading lady, and acted in comedies by F. Bohomolec before her retirement in 1767.