In enzymology, an alpha,alpha-phosphotrehalase (EC 126.96.36.199) is an enzyme that catalyzes the chemical reaction
alpha,alpha-trehalose 6-phosphate + H2
D-glucose + D-glucose 6-phosphate
Thus, the two substrates of this enzyme are alpha,alpha'-trehalose 6-phosphate and H2O, whereas its two products are D-glucose and D-glucose 6-phosphate.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is alpha,alpha-trehalose-6-phosphate phosphoglucohydrolase. This enzyme is also called phosphotrehalase. This enzyme participates in starch and sucrose metabolism.