Supriya Ghosh (Editor)

5 Ethynyl 2' deoxyuridine

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Abbreviations  EdU
5-Ethynyl-2'-deoxyuridine httpsuploadwikimediaorgwikipediacommonsthu

5-Ethynyl-2ยด-deoxyuridine (EdU) is a thymidine analogue which is incorporated into the DNA of dividing cells and is used to assay DNA synthesis in cell culture. At high doses it can be cytotoxic.

Detection

EdU is detected with a fluorescent azide which forms a covalent bond using click chemistry. Unlike the commonly used bromodeoxyuridine, EdU detection requires no heat or acid treatment.

References

5-Ethynyl-2'-deoxyuridine Wikipedia


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