4 methyleneglutamate—ammonia ligase

In enzymology, a 4-methyleneglutamate—ammonia ligase (EC 6.3.1.7) is an enzyme that catalyzes the chemical reaction

ATP + 4-methylene-L-glutamate + NH₃ ⇌ AMP + diphosphate + 4-methylene-L-glutamine

The 3 substrates of this enzyme are ATP, 4-methylene-L-glutamate, and NH₃, whereas its 3 products are AMP, diphosphate, and 4-methylene-L-glutamine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). This enzyme is also called 4-methyleneglutamine synthetase. This enzyme participates in c5-branched dibasic acid metabolism.