The 2016-17 Welsh Football League Division Two will see the following 16 teams participating:
AFC Llwydcoed
AFC Porth
Aberbargoed Buds (relegated from Welsh Football League Division One)
Aberdare Town (relegated from Welsh Football League Division One)
Abergavenny Town (promoted from Welsh Football League Division Three)
Ammanford
Briton Ferry Llansawel (demoted from Welsh Football League Division One)
Croesyceiliog
Cwmamman United
Dinas Powys
Garden Village (relegated from Welsh Football League Division One)
Llanelli Town
Newport City (renamed from Llanwern FC)
Pontardawe Town
Pontypridd Town (promoted from Welsh Football League Division Three)
West End
