2 methylisocitrate dehydratase

In enzymology, a 2-methylisocitrate dehydratase (EC 4.2.1.99) is an enzyme that catalyzes the chemical reaction

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate ⇌ (Z)-but-2-ene-1,2,3-tricarboxylate + H₂O

Hence, this enzyme has one substrate, (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate, and two products, (Z)-but-2-ene-1,2,3-tricarboxylate and H₂O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]. This enzyme is also called (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase. This enzyme participates in propanoate metabolism.