2 acetolactate mutase

In enzymology, a 2-acetolactate mutase (EC 5.4.99.3) is an enzyme that catalyzes the chemical reaction

Hence, this enzyme has one substrate, 2-acetolactate, and one product, 3-hydroxy-3-methyl-2-oxobutanoate.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is 2-acetolactate methylmutase. Other names in common use include acetolactate mutase, and acetohydroxy acid isomerase. This enzyme participates in valine, leucine and isoleucine biosynthesis. It employs one cofactor, ascorbate.