|Champion Venus Williams|
Final score 6–1, 4–6, 6–4
|Runner-up Serena Williams|
The 1999 Lipton Championships – Women's Singles was the women's singles event of the fifteenth edition of the tennis tournament played at Miami, United States. It is the third WTA Tier I tournament of the year, and part of the US Spring tennis season. Venus Williams was the defending champion, and she successfully defending her title by defeating her sister Serena in the final 6–1, 4–6, 6–4.
All seeds received a bye to the second round.
- Christína Papadáki