1999 Estoril Open – Women's Singles

The 1999 Estoril Open – Women's Singles was the singles event of the first edition of the WTA Tour Estoril Open; a WTA Tier IV tournament and the most prestigious women's tennis tournament held in Portugal. This tournament was part of the ITF Circuit last year, and it was won by Barbara Schwartz. Schwartz was eliminated in the second round of this year's event.

Katarina Srebotnik won in the final 6–3, 6–1 against Rita Kuti-Kis.