16S rRNA pseudouridine516 synthase

16S rRNA pseudouridine⁵¹⁶ synthase (EC 5.4.99.19, 16S RNA pseudouridine⁵¹⁶ synthase, 16S PsiI⁵¹⁶ synthase, 16S RNA Psi⁵¹⁶ synthase, RNA pseudouridine synthase RsuA, RsuA, 16S RNA pseudouridine 516 synthase) is an enzyme with systematic name 16S rRNA-uridine⁵¹⁶ uracil mutase. This enzyme catalyses the following chemical reaction

The enzyme is specific for uridine⁵¹⁶ in 16S rRNA.