(S) canadine synthase

In enzymology, a (S)-canadine synthase (EC 1.14.21.5) is an enzyme that catalyzes the chemical reaction

(S)-tetrahydrocolumbamine + NADPH + H⁺ + O₂ ⇌ (S)-canadine + NADP⁺ + 2 H₂O

The 4 substrates of this enzyme are (S)-tetrahydrocolumbamine, NADPH, H⁺, and O₂, whereas its 3 products are (S)-canadine, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and the other dehydrogenated. The systematic name of this enzyme class is (S)-tetrahydrocolumbamine,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming). Other names in common use include (S)-tetrahydroberberine synthase, and (S)-tetrahydrocolumbamine oxidase (methylenedioxy-bridge-forming). This enzyme participates in alkaloid biosynthesis i. It employs one cofactor, heme-thiolate(P-450).