(3-Aminopropyl)triethoxysilane (APTES) is an aminosilane frequently used in the process of silanization, the functionalization of surfaces with alkoxysilane molecules. As well as the covalent attaching of organic film on metal oxides like SiO2, TiO2, and so on.
APTES can be used to covalently bond thermoplastics to poly(dimethylsiloxane) (PDMS). Thermoplastics are treated with oxygen plasma to functionalize surface molecules, and subsequently coated with an aqueous 1% by volume APTES solution. PDMS is treated with oxygen plasma and placed in contact with the functionalized thermoplastic surface. A stable, covalent bond forms within 2 minutes.
Use with cell cultures
APTES-functionalized surfaces have been shown to be nontoxic to embryonic rat cardiomyocytes in vitro. Further experimentation is needed to evaluate toxicity to other cell types in extended culture.
APTES is a toxic compound with an MSDS health hazard score of 3. APTES fumes are destructive to the mucous membranes and the upper respiratory tract, and should be used in a fume hood with gloves. If a fume hood is not available, a face shield and full face respirator must be implemented. The target organs of APTES are nerves, liver and kidney.